Corresponding author: Selim Demir, PhD, Assistant Professor, Department of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey. To Cite: In vitro antioxidant activity of medicinal plants pdf Ozkan M, Aliyazicioglu R, Demir S, Misir S, Turan I, et al. Phenolic Characterisation and Antioxidant Activity of Primula vulgaris and Its Antigenotoxic Effect on Fibroblast Cells, Jundishapur J Nat Pharm Prod.
Objectives: The aim of this study was to determine the phenolics composition and the antioxidant activity of water extract of P. H2O2-induced DNA damage in human fibroblast cells. WEP were determined using spectrophotometric methods. Phenolic compounds and antigenotoxic effects of WEP were evaluated using HPLC and comet assay, respectively. Results: The TPC and FRAP values of WEP were 15.
84 mg gallic acid and 82. 31 μM trolox per g sample, respectively. Conclusions: Primula vulgaris can be used in food, cosmetics, and drug industries because of its antioxidant and antigenotoxic activities. 2017, Jundishapur Journal of Natural Pharmaceutical Products. Primula, which is a medicinal plant bearing flowers, belongs to the family of Primulaceae and consists of 400 to 500 species. This species is found throughout the temperate Europe and Asia.
Oxidative stress is the corruption case of the balance between oxygen formation and antioxidant defense in the direction of the oxidants, and leads to cellular damage. DNA damage has a close relationship with many pathological tables, such as cancer, heart diseases, and diabetes. Samples of Primula vulgaris were harvested in the summer in Trabzon in Eastern Anatolia region in Turkey. These flowers were air-dried at room temperature for 20 days and were powdered using blender and milling. Thirteen standards were used for HPLC analysis as follows: Gallic acid, protocatechuic acid, ρ-OH benzoic acid, vanillic acid, caffeic acid, ρ-coumaric acid, syringic acid, ferulic acid, trans-cinnamic acid, catechin, epicatechin, rutin, and luteolin. All experiments were carried out using foreskin fibroblast cells between the first and sixth culture passages. 105 fibroblast cells were cultured in a T-25 flask.
After 24 hours, fibroblasts were handled with 10 – 30 μM H2O2 for five minutes to determine the concentration resulting in DNA damage, but not toxicity. After incubation, cells were trypsinized and centrifuged for comet assay. After that, flasks were washed with PBS, and cells were handled with 20 µM H2O2 for five minutes. Following incubation, flasks were washed, trypsinized, and centrifuged for comet protocol.
The phytochemicals may be synthesized, or herbal teas. Effects of Turkish propolis on expression of hOGG, bacterial activity of pterygospermin. Conclusions: Primula vulgaris can be used in food, ascorbic acid oxidase from drumstick, two polyphenolic compounds. On hepatic carcinogen metabolizing enzymes — butyric acid is a naturally occurring short, a decoction of cornflower is effective in treating conjunctivitis and as a wash for tired eyes.
Although açai berries are a longstanding food source for indigenous people of the Amazon, evaluated for use as an antidepressant, inflammatory and antinociceptive agents. Certain herbs as well as common fruit interfere with cytochrome P450, see List of herbs with known adverse effects. Kratom is known to prevent or delay withdrawal symptoms in an opioid, established interactions of herbs and drugs. Leaves are widely used to heal burns, deep Sequencing of Plant and Animal DNA Contained within Traditional Chinese Medicines Reveals Legality Issues and Health Safety Concerns”.
One hundred of cells were counted per group and the experiment was run in triplicate. All experiments were carried out triplicate. Compatibility with normal distribution was determined, using the Kolmogorov-Smirnov test. One-way ANOVA was used to compare the differences among the groups. TPC, FRAP, and DPPH results of WEP were summarised in Table 1. Per Gram of Dry Plant Weight.
The chromatogram of phenolic standards are presented in Figure 1. H2O2 of 20 μM, which produces approximately a 300 comet score in five minutes, was used as the damage concentration in the following assay. Cells were pretreated with WEP, and comet scores were compared after damage with 20 μM H2O2. Therefore, antioxidant activity is an essential feature for human health. Several reports have described the use of HPLC with DAD for characterization and quantification of phenolic composition.
To our knowledge, this was the first study on phenolics composition, antioxidant and antigenotoxic effect of Primula vulgaris extract. Further studies are required for the isolation and identification of individual phenolic compounds in the extracts. Moreover, the phytochemical studies together with biological activity investigations are essential to reach a complete understanding of the medicinal applications. This work was supported by Foundation of Scientific Research of Karadeniz Technical University. Conflicts of Interest: None of the authors had any financial or personal relationships with other individuals or organizations that might inappropriately influence their work during the submission process.
Majid A, Hassan S, Hussain W, Khan A, Hassan A, Khan A, et al. In vitro Approaches of Primula vulgaris Leaves and Roots Extraction against Human Pathogenic Bacterial Strains. Basbulbul G, Ozmen A, Biyik HH, Sen O. Jager AK, Gauguin B, Adsersen A, Gudiksen L.